Microfluidic jet injection for delivering macromolecules into cells.
نویسندگان
چکیده
We present a microfluidic based injection system designed to achieve intracellular delivery of macromolecules by directing a picoliter-jet of a solution towards individual cells. After discussing the concept, we present design specification and criteria, elucidate performance and discuss results. The method has the potential to be quantitative and high throughput, overcoming limitations of current intracellular delivery protocols.
منابع مشابه
Orientation of biomolecular assemblies in a microfluidic jet
We have investigated multilamellar lipid assemblies in a microfluidic jet, operating at high shear rates of the order of 107 s−1. Compared to classical Couette cells or rheometers, the shear rate was increased by at least 2–3 orders of magnitude, and the sample volume was scaled down correspondingly. At the same time, the jet is characterized by high extensional stress due to elongational flow....
متن کاملDesign and Manufacturing of Jet to free Stream Simulator to Experimental Study of Interaction of Oblique Jet in Crossflow
The study of interactions of jet into cross flow at different longitudinal and transverse angles of jet was studied. The following components were designed and constructed: a low velocity wind tunnel to produce the uniform flow, a flat plate with a traverse injection system to simulate the jet injection, and a spatial rake to measure the total pressure. The tests were carried out at longitudina...
متن کاملDesign and Manufacturing of Jet to free Stream Simulator to Experimental Study of Interaction of Oblique Jet in Crossflow
The study of interactions of jet into cross flow at different longitudinal and transverse angles of jet was studied. The following components were designed and constructed: a low velocity wind tunnel to produce the uniform flow, a flat plate with a traverse injection system to simulate the jet injection, and a spatial rake to measure the total pressure. The tests were carried out at longitudina...
متن کاملBiotin-mediated delivery of exogenous macromolecules into soybean cells.
We have demonstrated that attachment of biotin to a variety of macromolecules allows the uptake of those macromolecules into cultured soybean cells (Glycine max Merr cv Kent). Macromolecules that were nondestructively delivered into intact cells in large numbers (>10(6)/cell) by this technique include bovine insulin (M(r) about 5,700), bovine ribonuclease (M(r) about 14,000), human hemoglobin (...
متن کاملFabrication of microfluidic hydrogels using molded gelatin as a sacrificial element.
This paper describes a general procedure for the formation of hydrogels that contain microfluidic networks. In this procedure, micromolded meshes of gelatin served as sacrificial materials. Encapsulation of gelatin meshes in a hydrogel and subsequent melting and flushing of the gelatin left behind interconnected channels in the hydrogel. The channels were as narrow as approximately 6 microm, an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of micromechanics and microengineering : structures, devices, and systems
دوره 23 شماره
صفحات -
تاریخ انتشار 2013